Murashige and Skoog Medium: A Plant Growth Medium for Cell Culture

Overview

Murashige and Skoog medium (MSO or MS0) is a plant growth medium widely used in laboratories for cultivating plant cell cultures.

Background

* MSO supports the growth of both dicots and monocots. * It is known for its ability to facilitate plant regeneration.

Major Breakthrough

* In the 1960s and 1970s, significant advances were made in the development of plant media. * A notable contribution was the publication by Murashige and Skoog in 1962, which introduced MSO.

Success in Plant Regeneration

* Experiments have demonstrated successful shoot regeneration using MSO supplemented with 0.5 mLL of 6-Benzylaminopurine (BAP) and 0.2 mgL of NAA (Naphthalene Acetic Acid).